Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.). Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.